Become a Facility User

Manufacturer

Agena Bioscience

Model

MassArray System with Epityper DNA Methylation Analysis

Location

ASRC 4th Floor Rm 4.114

MassArray System

Agena Bioscience MassArray System

The MassArray System is a complete system for discriminating methylated vs. non-methylated DNA; includes software, reagents, and MALDI-TOF mass spectrometry for detection and quantification.

EpiTYPER biochemistry starts with bisulfite treatment of genomic DNA, followed by PCR amplification of target regions. The reverse primers contain a T7 promoter tag. Next, in vitro RNA transcription is performed, followed by base-specific RNA cleavage. Finally, the cleavage products are analyzed using MALDI-TOF mass spectrometry (MassARRAY Analyzer). The methylated and non-methylated cytosine residues in the original genomic DNA are easily distinguished using EpiTYPER Software.

Important Notes

  1. Please fill sample submission form and attach to samples.
  2. Please check out as supplies in Badger (link http://cuny1.badgerlms.com/badger/News.html) to submit samples. Please make sure to check both SpectroChIPs and amplicons.
  3. Users are responsible for designing primers and PCR amplification of the target. The core only processes the samples after PCR of the amplicons.
  4. Samples must be submitted within one week after PCR amplification. Successful PCR is KEY to successful MassARRAY analysis. A document containing agarose gels showing the PCR results must be co-submitted.
  5. Results will be returned to users via SFTP.

Features

  • Interrogates 10s-100s of samples and CpG sites in amplicons from 200 – 600 bp and detect down to 5% differences in methylation.
  • The EpiTYPER technology includes online assay design software, robust biochemistry steps, and precise detection of reaction products using highly quantitative mass spectrometry.
  • Can also be used for other nucleic acid analysis methods, including SNP and somatic mutation detection.

Available Formats

  • 96 well Chips

Key Supported Applications

  • Targeted DNA methylation
  • Genotyping and mutation detection