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Manufacturer

Agena Bioscience

Model

MassArray System with Epityper DNA Methylation Analysis

Location

ASRC 4th Floor Rm 4.114

Agena Bioscience MassArray System

Coming Soon

The MassArray System is a complete system for discriminating methylated vs. non-methylated DNA; includes software, reagents, and MALDI-TOF mass spectrometry for detection and quantification.

EpiTYPER biochemistry starts with bisulfite treatment of genomic DNA, followed by PCR amplification of target regions. The reverse primers contain a T7 promoter tag. Next, in vitro RNA transcription is performed, followed by base-specific RNA cleavage. Finally, the cleavage products are analyzed using MALDI-TOF mass spectrometry (MassARRAY Analyzer). The methylated and non-methylated cytosine residues in the original genomic DNA are easily distinguished using EpiTYPER Software.

Features

  • Interrogates 10s-100s of samples and CpG sites in amplicons from 200 – 600 bp and detect down to 5% differences in methylation.
  • The EpiTYPER technology includes online assay design software, robust biochemistry steps, and precise detection of reaction products using highly quantitative mass spectrometry.
  • Can also be used for other nucleic acid analysis methods, including SNP and somatic mutation detection.

Available Formats

  • 96 well Chips

Key Supported Applications

  • Targeted DNA methylation
  • Genotyping and mutation detection